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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 13;193(2):909–920. doi: 10.4049/jimmunol.1400666

Figure 1. Both Lyn and Ets1 regulate plasma cell differentiation in a B cell-intrinsic fashion.

Figure 1

(A) Lyn-deficient bone marrow chimeras were generated by mixing wild-type congenic B6.IgMa bone marrow with IgMb+ C57BL/6 genetic background Lyn+/+ or Lyn−/− bone marrow and transferring into irradiated B6.IgMa recipients. Ets1-deficient bone marrow chimeras were generated by mixing wild-type congenic B6.IgMa fetal liver cells from E16.5 day embryos with C57BL/6 IgMb+ Ets1+/+ or Ets1−/− fetal liver cells (also from E16.5 day embryos) and transferring into irradiated Rag2−/− recipients. (B) Six to eight weeks later, the frequency of B220loCD138hi plasma cells among IgMa (wild-type) and IgMb (wild-type or Lyn−/−) expressing cells was determined by flow cytometry. In Ets1-deficient chimeras, IgMa (wild-type) and IgMb (wild-type or Ets1−/−) plasma cells were enumerated with allotype-specific ELISPOT. Two separate groups of 3-4 chimeric mice of each genotype were generated and analyzed independently. *p<0.05, **p<0.01.