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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 13;193(2):909–920. doi: 10.4049/jimmunol.1400666

Figure 6. SHP1 is the major phosphatase regulating Ets1 levels in B cells.

Figure 6

(A) Western blot for Ets1 and GAPDH with B cell lysates from wild-type or motheaten viable mice with heterozygous (mev/me+) or homozyogous (mev/mev) mutations in the SHP1 phosphatase (shown are representative results from one of 3 separate experiments, n=4 mice of each genotype in total analyzed) (B) RT-qPCR analysis of Ets1 mRNA in B cells from mev mice (n=3 for each genotype). (C) Western blot for Ets1 and GAPDH with B cell lysates from wild-type, Ptpn6fl/fl (SHP1 floxed) and Ptpn6fl/fl mb1-Cre (SHP1 knockout) mice. Shown are the results of one of three independent experiments (with a total of 6 mice of each genotype analyzed). (D) RT-qPCR analysis of Ets1 mRNA in B cells from SHP1fl/fl and SHP1fl/fl mb1-Cre mice (n=4 mice of each genotype). (E) Western blot for Ets1 and GAPDH with B cell lysates from wild-type mice, Inpp5dfl/fl (SHIP1 floxed) or CD19-Cre Inpp5dfl/fl (SHIP1 knockout) mice. Shown are the results of one of two independent experiments (with a total of 4 mice of each genotype analyzed). (F) RT-qPCR analysis of Ets1 mRNA in B cells from Inpp5dfl/fl or Inpp5dfl/fl mb1-Cre mice (n=4 for each genotype). **p< 0.01, ***p<0.001.