RT-PCR analysis of IL-2, IL-4, IL-5, IFN-γ, and β2-microglobulin mRNA levels in the skin biopsies from skin sites of mice sensitized with OVA or saline. Levels were normalized to β2-microglobulin. The filled bars represent the OVA-sensitized mice, and open bars represent the saline-sensitized mice. Pooled results of experiments using four OVA-sensitized mice and four saline-sensitized controls in duplicate for each strain of mice. IL-4–/– and IFN-γ–/– mice are in BALB/c background, and IL-5 –/– mice are in C57BL/6 background. (a) IL-2 levels. (b) IL-4 levels. (c) IL-5 levels. (d) IFN-γ levels. Bars represent mean ± SE. *P < 0.05 compared with WT controls.