Table 2.

Nutrient consumption, metabolite synthesis, conversion nutrient to metabolite factors and other characteristic parameters for each batch experiment

Bioreactor	DO (%)	NaHCO3 addition	Xmax–H (106cell/mL)	Xmax–S (106cell/mL)	μmax (day−1)	Reference time (day)	(pg/cell)	(pg/cell)	(pg/cell)	(pg/cell)	(pg/cell)	(mol/mol)	(mol/mol)	(mol/mol)
Bioflo 110	10	Without	1.85	1.73	1.30	1.78EEP	197	1317	10	14	1094	0.05	0.59	1.68
						2.23MCC	238	1287	14	34	1049	0.06	1.17	1.65
	30	Without	3.63	2.95	1.93	2.23EEP	139	655	12	14	536	0.09	0.85	1.65
						2.82MCC	130	696	11	13	565	0.09	0.84	1.64
		With	4.94	3.94	1.52	2.24EEP	102	533	3	8	378	0.03	0.60	1.44
						2.78MCC	93	681	3	9	440	0.03	0.78	1.31
	50	Without	4.34	3.73	1.55	2.16EEP	147	539	7	8	372	0.05	0.42	1.40
						2.83MCC	115	581	7	8	368	0.06	0.58	1.28
		With	4.24	3.68	1.68	2.30EEP	141	654	6	4	559	0.05	0.25	1.73
						2.88MCC	116	988	7	6	752	0.06	0.44	1.54
Celligen	10	Without	4.59	3.51	1.81	1.98EEP	111	692	7	0.2	492	0.06	0.01	1.44
						2.95MCC	100	864	6	5.0	604	0.06	0.04	1.42
	30	Without	3.07	2.97	1.69	2.10EEP	181	1045	27	5.5	919	0.15	0.25	1.78
						2.80MCC	146	1059	19	4.4	914	0.13	0.25	1.75
	50	Without	3.16	3.35	1.49	2.01EEP	204	1090	22	22	902	0.11	0.88	1.67
						2.67MCC	223	1619	25	26	1335	0.11	0.94	1.67

All factors were calculated using as initial moment, the time when the first sample of batch experiments was taken. Reference times, end of exponential phase (EEP) and maximum cell concentration (MCM), were used to define the end of time interval to calculate factors. Gln, Glc, Glu, NH₄ and Lac represent glutamine, glucose, glutamate, ammonium and lactate, respectively. X_max–H and X_max–S represent maximum viable cell concentration determined by hemocytometer and total cell concentration by Millipore^® Scepter handheld automated cell counter, respectively