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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cell. 2014 Jul 3;158(1):143–156. doi: 10.1016/j.cell.2014.05.035

Figure 7. Jub recruits Wts to adherens junctions.

Figure 7

A) Wing disc expressing GFP:Wts (green), and stained for E-cad (red). B) Wing disc expressing Jub:GFP (green), and stained for Wts:V5 (red). C) Western blots and quantitation of co-immunoprecipitation of Wts with Jub:GFP from third instar wing disc lysates (200 discs per lane). Lysates were made on discs dissected from nub-Gal4 Jub:GFP, nub-Gal4 Jub:GFP UAS-sqh.EE, or w-. Upper two panels show amounts in lysates (Input), lower two panels show the amounts precipitated by GFP-TrapA beads. Some non-specific precipitation of Wts occurs, but precipitation from animals expressing Jub:GFP is consistently greater. Histogram shows the average ratio of Wts in input over that in IP from three biological replicates, normalized to the ratio in nub-Gal4 Jub:GFP,. For both input and IP blots, the level of Wts was normalized to the Jub:GFP level. D) Wing disc expressing GFP:Wts (green), with jub mutant clones marked by absence of arm-lacZ (blue). E,F) Wing discs expressing GFP:Wts (green) and en-Gal4 UAS-dcr2 UAS-RFP (blue) and E) UAS-rok-RNAi or F) UAS-sqh.EE, and stained for E-cad (red). G) Quantitation of Wts:GFP and E-cad at adherens junctions in discs of en-Gal4 UAS-dcr2 UAS-RFP plus, where indicated, UAS-rok-RNAi or UAS-sqh.EE, represented as the mean intensity of junctional fluorescence in P compartment regions divided by the mean intensity in A compartment regions (N= 5 discs per genotype, with 5 measurements per disc). H) Wing disc expressing GFP:Wts (green), and stained for Ex (magenta) and Sav (red), as indicated. Panels marked –z show vertical sections, white arrow points to location of a GFP:Wts puncta. I) Model for regulation of Hippo signaling by cytoskeletal tension through Jub, see text for details. Error bars show sem. See also Fig. S6.