Figure 5.

B6.SLE T_eff accelerate atherosclerosis independent of the source of T_reg. (A) Representative images of Oil Red O stained sections of the aortic root (top) and quantification of the total lesion area based on the Oil Red O staining (bottom). (B) Representative images of immunofluorescent staining for CD4 ⁺ cells (red), with 4′, 6-diamidino-2-phenylindole (DAPI) staining (blue) for nuclei (top) and quantification of the proportion of total cells that were shown to express CD4 (bottom). (C) Whole splenocytes from recipients were cultured with or without anti-CD3/CD28 stimulation for 5 h and then subjected to intracellular staining for IL-17. The percentage of cytokine positive cells was measured by flow cytometry. The percent of IL-17⁺ cells in the CD4⁺FoxP3⁻ (left) and CD4⁺FoxP3⁺ (right) populations. (D) IL-10R expression was measured on T_eff and T_reg from recipients. Cells were gated based on CD4⁺ expression, then separated into T_eff and T_reg populations. IL-10R staining was measured by flow cytometry. Data reflect n=6 mice per group. *^, ** and *** indicate significance at p<0.05, 0.01 and 0.001, respectively. SLE, systemic lupus erythematosus.