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Monte Carlo simulation of F factor for heterodyne Fourier confocal FLIM compared to single frequency homodyne frequency domain lifetime method, at 100% and 80% excitation modulation depth. For 1~4 ns lifetimes Fourier confocal FLIM requires 5,000~1,0000 photons to achieve 10% lifetime accuracy. Homodyne lifetime F-factor curves are recalculated with same parameters as in Ref [10].
