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. 2014 Jan;108(1):21–29. doi: 10.1179/2047773213Y.0000000115

Table 3. Pathogenicity profiles of Listeria species recovered from udder raw fresh whole milk of goat and sheep.

Source of milk samples Listeria species Pathogenicity profile PCR profile of virulence associated genes
CAMP (±) with S/R PI-PLC DLABN DAPN Anton's eye test Mice lethality Chick-embryo lethality Vero cell ingestion assay Biofilm
Christensen's tube Microtiter plate assay hlyA plc</emph>B actA iap
L. monocytogenes (ATCC 7494) ± + + + + + Strong Strong (OD  =  0.158) + + + +
Goat L. innocua + + Weak Strong (OD  =  0.159) _ _ _ _
L. seeligeri + + Moderate Strong (OD  =  0.221) + _ _ _
L. welshimeri + + Moderate Very strong OD  =  0.112) _ _ _ _
Sheep L. innocua + + Moderate Strong (OD  =  0.107) _ _ _ _
L. grayi + + Weak Strong (OD  =  0.127) _ _ _ _
L. grayi + + Moderate Strong (OD  =  0.126) _ _ _ _

CAMP: Christie, Atkins, Munch-Petersen test; S/R: Staphylococcus aureus/Rhodococcus equi; PI-PLC: phosphatidylinositol-specific phospholipase C; DLABN: DL-alanine-beta-naphthylamide HCl; DAPN: d-alanine-p-nitroanilide; OD: optical density; OD < 0.1  =  weak; OD 0.1  =  strong; OD > 0.1  =  very strong.