Table 1.

Primer sequences used for In-Fusion cloning.

Fragment	Forward primer	Reverse primer
−(6.1–0.8)	TTGGCGCCTCCCGCGGCCGCgatgagcccggcgtcccatctt	GTTTGGATGTTCGCGGCCGCcctttctaagaggcctggttctgg
−(6.1–3.7)	TTGGCGCCTCCCGCGGCCGCgatgagcccggcgtcccatctt	GTTTGGATGTTCGCGGCCGCtctctgagaagctgggagtctg
−(3.7–0.8)	TTGGCGCCTCCCGCGGCCGCttgaatcatttgagaagtgagttt	GTTTGGATGTTCGCGGCCGCcctttctaagaggcctggttctgg
−(13.7–6.1)	TTGGCGCCTCCCGCGGCCGCggatccttctctgtaaagtagac	GTTTGGATGTTCGCGGCCGCgtcgactcaggctgagaatt
−(13.7–9.7)	TTGGCGCCTCCCGCGGCCGgatccttctctgtaaagtagac	GTTTGGATGTTCGCGGCCGCgtgctgcgagccaactgagcc
−(9.7–6.1)	TTGGCGCCTCCCGCGGCCGCcatcaggttagctggaac	GTTTGGATGTTCGCGGCCGCgtcgactcaggctgagaatt
−(7.9–7.6)	TTGGCGCCTCCCGCGGCCGCggcaagccctgtgtctccct	GTTTGGATGTTCGCGGCCGCcccccctggaaacagggtgt
−(7.4–6.6)	TTGGCGCCTCCCGCGGCCGCcacacacacccctgcacaac	GTTTGGATGTTCGCGGCCGCaggcactgggatcaggc
−(13.0–12.5)	TTGGCGCCTCCCGCGGCCGgatggagccgctgattctga	GTTTGGATGTTCGCGGCCGCggggagcagggttggaatcc
−(26.9–24.0)	TTGGCGCCTCCCGCGGCCGCagaccaaggtctgcaagt	GTTTGGATGTTCGCGGCCGCggaactaacacttgtcctg

Capital letters indicate the sequences that are homologous to the vector.