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. 2014 Jul 7;4:5596. doi: 10.1038/srep05596

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(A) ARP2 expression after culture for 9 h. In the control group, ARP2 was expressed mainly at the membrane, whereas ARP2 expression was significantly reduced in oocytes that were injected with WASH1 MO or a Strumpellin antibody. Green, ARP2; blue, chromatin. Bar = 20 μm. (B) Average ARP2 fluorescence intensities were determined in mouse oocytes after injection of WASH1 MO or a Strumpellin antibody. *, significant difference (p<0.05). (C) Cropped gels were used for our western blot results. Western blot results showed that ARP2 expression was significantly reduced after injection with WASH1 MO. ARP2 molecular mass is 43 kDa and that of α-tubulin is 55 kDa. Relative ARP2 or α-tubulin staining intensity was assessed by densitometry.