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Time series. Aged hMSC of all three donors were stimulated with osteogenic differentiation media (ODM) for 21 days. Media change was carried out every 96 hours. Micrographs were taken (A) and quantification of alizarin red was performed (B) at the designated time points. Starting with day 9 there was a significant increase in extracellular calcium deposition compared to standard growth media (aMEM) controls. The graph shows mean values and standard deviation error bars.
