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. 2014 Jun 24;58(2):2254. doi: 10.4081/ejh.2014.2254

Figure 6.

Figure 6.

Strategic scheme of choice of the antibodies combinations to be used for each new experiment. It should be adapted for each muscle and each condition. The four antibodies BA-D5, SC71, BF-F3, RTD-9 should be systematically applied with laminin on serial sections. The first step is to choose the combination of antibodies for image analysis. In absence of MyHC IIb, (BF-F3 negative fibres), the BA-D5/SC71 combination should be used to discriminate type I, IIA, IIX and hybrid fibres. In absence of MyHC I (BA-D5 negative fibres), the SC71/BF-F3 combination should be used to discriminate type IIA, IIX, IIB and hybrid fibres. If these absences are not validated by the image-analysis, the combination BA-D5/SC71/BF-F3 should be used for fibre typing. Labeling with RTD-9 antibody is used only to validate the presence of MyHC IIx in IIX fibres identified by the absence of any labeling.