Strategic scheme of choice of the antibodies combinations to be used for each new experiment. It should be adapted for each muscle and each condition. The four antibodies BA-D5, SC71, BF-F3, RTD-9 should be systematically applied with laminin on serial sections. The first step is to choose the combination of antibodies for image analysis. In absence of MyHC IIb, (BF-F3 negative fibres), the BA-D5/SC71 combination should be used to discriminate type I, IIA, IIX and hybrid fibres. In absence of MyHC I (BA-D5 negative fibres), the SC71/BF-F3 combination should be used to discriminate type IIA, IIX, IIB and hybrid fibres. If these absences are not validated by the image-analysis, the combination BA-D5/SC71/BF-F3 should be used for fibre typing. Labeling with RTD-9 antibody is used only to validate the presence of MyHC IIx in IIX fibres identified by the absence of any labeling.