Figure 3.

Identification of miR29b in blood–brain barrier disruption by using RT²-qPCR microRNA array. (A) RT²-qPCR array determined altered expression of different microRNAs in BBB disruption model in bEnd.3 cells (treated with 100 μmol/L of homocysteine (Hcy)) as compared with control, and the results are represented through horizontal bar graph. Dotted inset is showing upregulation of miR29 family. (B) Bar graph representing qPCR analyses of individual miR29 family members (miR29a, miR29b1, miR29b2, and miR29c) in bEnd.3 cells treated with Hcy (100 μmol/L), and 5′-azacytidine (10 μmol/L). Data represent mean±s.e.m. *P<0.05, **P<0.005, ***P<0.0005 versus control; ^#P<0.05, ^##P<0.05, ^††P<0.005, ^†††P<0.0005 versus Hcy-treated cells; data analyzed from four independent experiments (n=4).