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. 2014 Jun 20;5:4181. doi: 10.1038/ncomms5181

Figure 7. Snf2h loss alters PC maturation.

Figure 7

(a) Snf2h (red) and Calbindin (green) co-labelling through the cerebellum from Snf2h cKO-Nes and control littermates at P7. Note the absence of Snf2h expression in mutant PCs. Scale bar, 20 μm. (b) Confocal Z-stacks through the cerebellar vermis from Snf2h cKO-Nes and control littermates at P7 immunolabelled for Calbindin (green). Roman numerals denote the corresponding lobules of the mammalian cerebellum. Scale bars, 100 μm (left panels); 20 μm (right panels). (c) Light microscopy Z-stacks of PCs through the cerebellar vermis stained with the Golgi–Cox method from Snf2h cKO-Nes mice and control littermates at P20. Scale bar, 10 μm. (d) Molecular layer size at P7 and P14 in Snf2h cKO-Nes and control littermates. (e) Quantification of pyknotic PC nuclei using toluidine blue reveals an ISWI-dependent modulation of PC survival in Snf2h cKO-Nes, Snf2l−/+::Snf2h cKO-Nes and cDKO-Nes mice. (f,g) PC apical arbor dendritic length measurements from the indicated genotypes at P20 or P30, respectively. For panels (d–g) **P<0.01, Student’s t-test, n.s., not significant, n=6. Values are presented as the mean±s.e.m. (h) Left panels: confocal Z-stacks through the cerebellum from P30 Snf2h cKO-PCP2 mice and control littermates co-immunolabelled for Snf2h (red) and calbindin (green). Arrows and bars denote mutant PC arbors that do not reach the pial surface (ps) (bars, arrows). Middle panels: higher magnification images of PCs (arrowheads) denote Snf2h+ staining in control but not mutant PCs. Right panels: pseudocolored Calbindin-ir (silver) denotes the atrophied dendritic arbor in mutant PCs (bars, arrows). Scale bar, 20 μm (left and rightmost panels); 10 μm (middle panels). For a–c and h, at least three mice from each genotype were used for evaluation.