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Effect of diffusates from elicited cotyledons on NO production by stimulated macrophages. Cells were cultured for 48 h with LPS (20 ng/mL) (square symbols) or LPS (20 ng/mL) plus IFNγ (56 IU/mL) (circle symbols) in the presence of plant diffusates collected at different times after Dpm (A) or SNP (B) inoculation. The concentrations of NO were expressed as nitrite. The points are the means ± SE of three independent experiments, each done in quadruplicate.
