Background-subtracted, color-coded ultrasound contrast images of the left ventricle in the short-axis view during coronary occlusion (A) and 4 hours after reperfusion (B, D to F). A, During coronary occlusion and injection of nontargeted microbubbles, there was a risk area (region between the arrows), corresponding to the left anterior descending (LAD) coronary artery territory. B, During reperfusion and injection of nontargeted micro-bubbles, there was homogeneous contrast enhancement of the myocardium. C, There was no infarction by triphenyl tetrazolium chloride staining. D, After injection of MBESEL, there was persistent contrast enhancement of the previously ischemic LAD coronary artery territory, not seen after injection of either of the MBCTL (E) or MBIgG (F).