Table I.
Primary antibodies used in this study
| Antibodies | Clone/Catalog # | Tissue Fixation | Antigen Retrieval |
|---|---|---|---|
| NKG2A | Epitomics (T3308) | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| Granzyme H | Sigma HPA029200 | PFA | 1 mM EDTA Buffer pH8.0 98°C 20 min |
| Granzyme B | Spring Bioscience E2580 | PFA | 1 mM EDTA Buffer pH8.0 98°C 20 min |
| IFN-γ | Abcam ab25101 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| IL-2Rβ (CD122) | Novus Biologicals NBP1-19140 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| HLA-E | MEM-E/06 ab3984 | PFA | 1 mM EDTA Buffer pH8.0 98°C 20 min |
| CD3 | AbD Serotech MCA 1477 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| CCL3/MIP-1α | Neomarkers RB-10489-P | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| CCL4/MIP-1β | R&D AF-271-NA | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| CCL5/RANTES | R&D AF-278-NA | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| Ki67 | Neomarkers RM9106 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| HLA-DR/DQ/DP | DAKO M0073 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| CD69 | Novacastra NCL-CD69 CH11 | SAFE | 1 mM EDTA Buffer pH8.0 98°C 20 min |
| CD38 | Vector VP-C348 SPC32 | SAFE | 1 mM EDTA Buffer pH8.0 98°C 20 min |
| IP10 | R&D AF-266-NA | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| CD107a | LifeSpan LS-B580 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| CD68 | DAKO KP1 M0814 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |
| Vimentin | Neomarkers RB-9063 | PFA | 10 mM Citrate Buffer pH6.0 98°C 20 min |