Figure 3.

Rescue of isolated E13 metanephric mesenchymes from apoptosis by TIMP-2. Metanephric mesenchyme involutes (a) and is replaced by apoptotic bodies (c) when cultured for 48 hours in tissue culture medium (MEM with 10% FCS). In contrast, the addition of rTIMP-2 (2 μg/mL) rescues metanephric mesenchymal cells from apoptosis (b). Compared with mesenchymes incubated in basal medium, few apoptotic bodies are found when rTIMP-2 is included (d). Scale bars in a and b: 125 μm. Scale bars in c and d: 10 μm.