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GDNF in metanephric mesenchymal cells that were rescued from apoptosis by incubation with TIMP-2. GDNF was detected by immunoblots in 6 freshly isolated E13 mesenchymes (0 hours) and in 6 mesenchymes maintained in culture by rTIMP-2 for 48 hours (48 hours + TIMP-2). In contrast, little reactivity remains in 6 untreated mesenchymes (48 hours). Standard is 25 ng of recombinant rat GDNF.
