Figure 8.

TIMP-2 enhances matrix deposition. E13 kidneys were cultured for 4 days with rTIMP-2 (2 μg/mL) and stained with antibodies to collagen IV. The entire ureteric tree is stained by collagen IV antibodies after rTIMP-2 treatment (b), including tip regions (arrows), while only proximal parts of the ureteric bud are obviously ensheathed by collagen IV in control kidneys (a). A few tips of the ureteric bud are indicated (arrow). (c) Similar to the effect of rTIMP-2, treatment of E13 kidneys with ilomastat (2 μM) resulted in matrix deposition throughout the ureteric tree. X-Y projection of serial cuts through the kidney. Scale bars: 100 μm.