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. 1999 May 1;103(9):1329–1335. doi: 10.1172/JCI4742

Figure 2.

Figure 2

DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. (a) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. (b) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.