Figure 6.

Pulse DS stimulation commits endothelial cells to ICAM-1 expression. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. (a) Cells were cultured for the indicated times with 50 μg/mL DS in defined media containing 1% FCS. (b) Cells were cultured as described for the indicated times followed by removal of the media, rinsing of cells 2 times with fresh media, and incubation for the duration of a 24-hour period with media lacking DS. Thus, each time point in graph (b) represents a total incubation time of 24 hours, with DS present for the indicated times. Data in a and b represent mean relative cell surface ICAM-1 expressed as mean OD/min ± SD for triplicate determinations.