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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cancer Res. 2014 Apr 25;74(13):3477–3488. doi: 10.1158/0008-5472.CAN-13-2639

Fig. 2. NCOA1 overexpression in mammary tumor cells enhances macrophage recruitment and CSF1 expression.

Fig. 2

A. Immunostaining of macrophages (brown color) with F4/80 antibody in Tg(Neu) (Neu) and Tg(NCOA1)×Tg(Neu) (NCOA1×Neu) mammary tumors. T, tumor area; M, macrophage. B. Macrophage numbers in Tg(Neu) and Tg(NCOA1)×Tg(Neu) tumors at early and late stages. Macrophages in 10-viewing fields at 400× magnification were counted on each tumor section. Five tumors were analyzed for each group. Data are presented as mean ± standard deviation (SD). *, p<0.05. C. qPCR measurement of CSF1 mRNA levels in individual Tg(Neu) and Tg(NCOA1)×Tg(Neu) mammary tumors. D. Adenovirus-mediated NCOA1 expression (Ad-NCOA1) in MCF-7 and MDA-MB-231 cells induced CSF1 expression as assayed by qPCR. Adenovirus-mediated GFP expression (Ad-GFP) served as a control.