Fig. 5.

S. mutans biofilm formation in the absence (black bars) and presence (grey bars) of known inhibitors of amyloid fibrillization, CR (10 µM) (a), ThS (100 µM) (b) and EGCG (1 mM) (c). Strains 3svf1, 1svf1, 5ST1 and 81D2 are clinical isolates. NG8 was used as the parent to generate the P1-deficient isogenic mutant PC3370 (Crowley et al., 1999). Error bars represent sem. In each case there was a significant decrease in biofilm formation in the presence of inhibitor at the P≤0.0001 level, except NG8 plus ThS (P≤0.0015) and 1svf1 plus CR (P≤0.0085). There was no significant difference between NG8 with CR and NG8 without CR at the P≤0.05 level.