Figure 10.

Uptake of ¹²⁵I-labeled lipoproteins by CHO cells transfected with the murine scavenger receptor class A type I. Confluent CHO cells expressing the murine scavenger receptor class A type I (white bars) or control vector-transfected parental CHO cells that lack the LDL receptor (black bars) were incubated for 5 hours at 37°C with 5 μg/mL of the indicated ¹²⁵I-labeled lipoproteins prepared as described in Methods. Cellular uptake of modified lipoproteins was then determined as described in Methods. Data represent the mean ± SD of triplicate determinations. Similar results were observed in 3 independent experiments. *P < 0.05 vs. control vector-transfected parental CHO cells; **P < 0.001 vs. control vector-transfected parental CHO cells. acLDL, acetylated LDL; LDL, native LDL; mSR-AI, CHO cells expressing the murine scavenger receptor class A type I; + NO₂-LDL, LDL modified by the complete MPO-H₂O₂-NO₂^- system; – NO₂-LDL, LDL modified by the complete system in the absence of NO₂^–; oxLDL, copper oxidized (5 hours) LDL.