Figure 5.

Time course of LDL conversion into a high-uptake form after exposure to the MPO-H₂O₂-NO₂^– system. [¹²⁵I]LDL (0.2 mg/mL) was incubated with isolated human MPO (30 nM), glucose (100 μM), and glucose oxidase (20 ng/mL) in sodium phosphate buffer (50 mM, pH 7.0) supplemented with DTPA (100 μM) for the indicated times in either the presence (+ NO₂^–; filled circles) or absence (– NO₂^–; open circles) of NO₂^– (500 μM) as described in Methods. Uptake of the modified lipoprotein by hMDMs (a) and thioglycollate-elicited MPMs (b) was then determined as described in Methods. Data represent the mean ± SD for triplicate determinations. Similar results were observed in 3 independent experiments.