Figure 9.

Concentration dependence of macrophage binding (a) and degradation (b) of LDL modified by the MPO-H₂O₂-NO₂^– system. (a) [¹²⁵I]LDL was modified in the presence of MPO and an H₂O₂-generating system (GGOx) in either the presence (+ NO₂^–; filled circles) or absence (– NO₂^–; open circles) of NO₂^– in sodium phosphate buffer (50 mM, pH 7.0) supplemented with DTPA (100 μM) as described in Methods. Thioglycollate-elicited MPM binding (a) and degradation (b) of the indicated concentrations of lipoproteins were then assessed as described in Methods. Data represent the mean of duplicate determinations (binding studies) or the mean ± SD of triplicate determinations (degradation studies). Similar results were observed in 3 independent experiments.