Table 1.
Properties of chimeric viruses.
| Virusa (clone) | C-prM junction | Geneb | Nucleotide change (chimeric virus numbering)b | Amino acid change (individual protein numbering)b | Phenotype in vero cells | ||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Mean peak titer (log10 PFU/mL) | Plaque size prior to adaptationc,d | Plaque size after adaptationc | |||||
| First-generation | |||||||
| JEV/DEN4 (CF-ME-0-13) | JEV | E | T1465C | F167S | 6.0 | Small | Large |
| E | A1900G | K312R | |||||
| E | T2254C | I430T | |||||
| NS4B | C7171T | P101L | |||||
| JEV/DEN4 (GG-ME-1-17) | DENV-4 | E | G1733C | Q264H | 6.6 | Small | Large |
| E | T2230C | I430T | |||||
| NS4B | C7147T | P101L | |||||
| 3′UTR | 10,485 A insert | N/A | |||||
| JEV/DEN4 (GG-ME-2-21) | WNV | E | A1671C | M240L | 6.1 | Small | Medium |
| E | A2391G | M480V | |||||
| NS3 | C5026T | S158L | |||||
| NS4B | C7171T | T105I | |||||
| JEV/DEN4Δ30 (CF-ME-0-14) | JEV | E | G1722T | V253F | 5.5 | Small | Small |
| NS2A | A4023G | M168V | |||||
| NS3 | G5170T | R202I | |||||
| NS4B | T7195C | V109A | |||||
| NS4B | C7588T | A240V | |||||
| JEV/DEN4Δ30 (GG-ME-1-24) | DENV-4 | NS3 | C5014T | S158L | 5.6 | Small | Small |
| NS4B | C7159T | T105I | |||||
| JEV/DEN4Δ30 (CF-ME-2-18) | WNV | E | T2043C | S364P | 5.3 | Small | Small |
| E | G2190A | G413R | |||||
| NS4B | T7192C | L112S | |||||
| Second-generation | |||||||
| JEV/DEN4 (B-1a) | DENV-4 | prM | G765A | E109K | 5.8 | Small | Small |
| NS3 | C5014T | S158L | |||||
| NS4B | C7159T | T105I | |||||
| JEV/DEN4 (C-14) | DENV-4 | E | T2230C | I430T | 6.0 | Small | Large |
| NS4B | C7147T | P101L | |||||
| JEV/DEN4 (AC-7a) | DENV-4 | E | G1733C | Q264H | 6.6 | Large | Large |
| E | T2230C | I430T | |||||
| NS4B | C7147T | P101L | |||||
| JEV/DEN4 (D-5a) | DENV-4 | E | A1567G | K209R | 6.0 | Small | Medium |
| NS4B | T7180C | L112S | |||||
| JEV/DEN4Δ30 (B-2a) | DENV-4 | prM | T936C | Y166H | 5.0 | Small | Small |
| NS3 | C5014T | S158L | |||||
| NS4B | C7159T | T105I | |||||
| JEV/DEN4Δ30 (D-6a) | DENV-4 | NS3 | G4667T | M42I | 3.9 | Small | Small |
| NS4B | T7180C | L112S | |||||
a
All viruses are ME chimeric viruses (CME chimeric viruses were not viable). ME has been omitted from the virus names.
b
Engineered mutations introduced into the second generation viruses are shown in bold.
c
Plaque size was measured on day 6 post-infection: small is <1 mm, medium is 1–2 mm, and large is >2 mm. Vero-adapted rJEV India/78 (3 days post-infection) and rDEN4 produce large plaques. Vero-adapted rDENΔ30 produces medium plaques. JEV SA14-14-2 (3 days post-infection) produces small plaques.
d
Plaque size on Vero cells directly after recovery of virus and prior to terminal dilutions on Vero cells.