Table 2.
Virulence in weanling and suckling mice.
| Virus | Clone | C-prM junction | Weanling mice (n = 6/dose)
|
Suckling mice (n = 10/dose)
|
||
|---|---|---|---|---|---|---|
| IP LD50 (PFU) | IC LD50 (PFU) | IC LD50 (PFU) | Median survival time (days)a | |||
| JEV India/78 | Wild-type | – | 40 | 0.6 | 1.5 | 4 |
| rJEV India/78 | Recombinant | – | 10 | 0.4 | 0.3 | 4 |
| JEV SA14-14-2 | Vaccine Strain | – | >10,000b | >10,000 | 0.3 | 7 |
| rDEN4 | Recombinant | – | >10,000 | >10,000 | >100 | >21c,d,e |
| rDEN4Δ30 | Vaccine strain | – | >10,000 | >10,000 | >100 | >21d,e |
| First-generation | ||||||
| JEV/DEN4 | CF-ME-0-13 | JEV | >10,000 | >10,000 | NT | NTg |
| JEV/DEN4 | GG-ME-1-17 | DENV-4 | >10,000 | >10,000 | NT | NT |
| JEV/DEN4 | GG-ME-2-21 | WNV | >10,000 | >10,000 | NT | NT |
| JEV/DEN4Δ30 | CF-ME-0-14 | JEV | >10,000 | >3000 | NT | NT |
| JEV/DEN4Δ30 | GG-ME-1-24 | DENV-4 | >10,000 | >4000 | NT | NT |
| JEV/DEN4Δ30 | CF-ME-2-18 | WNV | >10,000 | >2000 | NT | NT |
| Second-generation | ||||||
| JEV/DEN4 | B-1a | DENV-4 | >10,000 | >3200 | 22 | 10c,d,e |
| JEV/DEN4 | C-14 | DENV-4 | >10,000 | >5000 | 7 | 13d,e |
| JEV/DEN4 | AC-7a | DENV-4 | >10,000 | >10,000 | 0.8 | 8.5d,e,f |
| JEV/DEN4 | D-5a | DENV-4 | >10,000 | >10,000 | ≥1000 | 18.5d,e |
| JEV/DEN4Δ30 | B-2a | DENV-4 | >10,000 | >1000 | >1000 | >21d,e |
| JEV/DEN4Δ30 | D-6a | DENV-4 | >1600 | >160 | 17h | >21d,e |
a
Reported for the high dose IC (103 PFU).
b
All values shown with “>” sign indicate the highest dose administered.
c
>21 days is reported if the majority of mice survived until the end of the study.
d
Survival time is statistically different from rJEV India/78 by Log-rank (Mantel-Cox) test.
e
Survival time is statistically different from JEV SA14-14-2 by Log-rank (Mantel-Cox) test.
f
Survival time is statistically different from JEV/DEN4 C-14 by Log-rank (Mantel-Cox) test.
g
Not tested (NT).
h
Mortality did not follow a clear dose response.