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. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: Methods Mol Biol. 2010;647:95–111. doi: 10.1007/978-1-60761-738-9_5

Figure 1.

Figure 1

Determining the effect of LMB on the relative amounts of Smad2/3/4 in the cytoplasm and the nucleus. HaCaT cells were treated with and without 100 pM TGF-beta1 for one hour. This experiment was repeated with treatment of 20 ng/ml LMB, where LMB was applied 30 minutes prior to addition of TGF-beta. Cells were fractionated into cytoplasm and nuclear fractions and western blotted to determine the relative amounts of Smad2/3/4. Lamin A/C and Beta-actin were used as both loading controls and indicators of the purity of fractions.