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Effect of hexose phosphate substrates on 21 hydroxylase activity. Isolated intact adrenal microsomes were incubated with either 1 mM G6P, 1 mM F6P or 6.6 mM glucoseamine-6-phosphate (GlcN6P) and 21-hyroxylase activity was measured over 20 min in the presence of 1 mM NADP. In each experiment, NADP was at 1 mM. Results are expressed as a rate (pmol/mg) mean ± SD, n = 4.
