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Effect of various substrates and osmotic agents on 21-hydroxylase and 17-hydroxylase activity in porcine adrenal microsomes. Isolated intact adrenal microsomes were incubated for 15 min with G6P (1 mM), glucose (600 mM), GlucN6P (6.6 mM), sucrose (600 mM) and mannitol (600 mM) and the activities of 21-hydroxylase and 17-hydroxylase measured as per methods with 1 mM NADP. Results are given as mean ± SD, n = 4.
