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. 1999 Jun 15;103(12):1677–1684. doi: 10.1172/JCI5645

Figure 1.

Figure 1

Expression of CFTR and Cx45 in CFPAC-1 and PLJ-CFTR cells. (a) RT-PCR of mRNA isolated from both cell lines, as well as from T84, CAPAN, and SKHep1 cells, using primer pairs specific for CFTR (lanes 2–5) and Cx45 (lanes 6–10). Amplification products of the expected sizes for CFTR (expected size: 410 bp) were detected in T84 and PLJ-CFTR cells (lanes 1 and 4, respectively). No products were detected with these primers in CFPAC-1 and SKHep1 cells (lanes 3 and 5, respectively). mRNA for Cx45 (expected size: 309 bp) was detected in CAPAN, CFPAC-1, PLJ-CFTR, and SKHep1 cells (lanes 6, 8, 9, and 10, respectively), but not in T84 cells (lane 7). Molecular markers are shown in lanes 1 and 11 (b and c). Indirect immunofluorescence of CF and corrected pancreatic duct cells cultured on glass coverslips. Punctate labeling for Cx45 was detected in CFPAC-1 (b) and PLJ-CFTR (c) cell clusters at cell-cell contacts. Scale bar: 60 μm.