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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Cancer Res. 2014 Apr 16;74(12):3369–3377. doi: 10.1158/0008-5472.CAN-13-3216

Figure 4. Association of E2F1, GCN5, H3K9ac, and NER factors with UV-induced DNA damage is impaired in E2f1S29A/S29A cells.

Figure 4

Wild type (E2f1+/+) and E2f1S29A/S29A MEFs were exposed to 250 J/m2 of UVB and ChIP was performed 30 min post-irradiation with the indicated antibodies: (a) E2F1, (b) GCN5, (c) H3K9ac, (d) XPC, (e) XPA, and (f) control IgG. An ELISA was used to measure the amount of CPD in immunoprecipitated DNA. The relative enrichment of CPD in genomic DNA was calculated from two independent samples per group subjected to ELISA in triplicate.