Figure 3.

TP levels (a) and IL-5 concentration (b) measured in BAL fluid supernatants. Mice were treated with PBS or sensitized to SRW as described in Methods. BAL fluids were obtained from each group of mice (n = 4–5) at 48 hours after last intranasal challenge with either PBS or SRW. TP levels (a) were detected by the Bradford method. A significant increase in TP was present in both HLA-DQ6 and HLA-DQ8 mice compared with PBS-treated counterparts (P < 0.01 and P < 0.05, respectively). Levels of IL-5 were determined by ELISA (b). There was no detectable IL-5 in BAL from PBS-treated mice. SRW sensitization and challenge resulted in the increase of IL-5 content in BAL of HLA-DQ mice. There was no detectable IL-5 in BAL of SRW-sensitized H-2Aβ⁰ mice or anti-DQ–treated SRW-sensitized HLA-DQ6 mice. Data are expressed as mean ± SEM from 1 of 3 independent experiments.