Figure 7.

Inhibition of SRW-specific HLA-DQ spleen MNC (a) and BAL cell (b) proliferative response. (a) Spleens were harvested from mice at 48 hours after intranasal challenge with SRW, and spleen MNCs were cocultured in vitro with allergenic extract in the presence of the indicated mAb’s. The data represent the percentage of inhibition of [³H]thymidine incorporation in triplicate cultures with SRW for 3 mice per experimental group from 1 of 2 independent blocking experiments. (b) BAL fluids were obtained from mice on day 5 after challenge, and BAL cells were cocultured with SRW and mAb for 3 days. The values displayed are the result of pooled BAL cells from 5 mice per strain and are representative of 2 analyses. Changes in cpm values for cultures with SRW alone (0% inhibition) are indicated.