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. Author manuscript; available in PMC: 2014 Jul 7.
Published in final edited form as: J Biol Chem. 2007 Jun 27;282(35):25842–25851. doi: 10.1074/jbc.M703474200

FIGURE 6. Evaluation of SFK signaling importance for GDNF-regulated expression of self-renewal genes in cultured SSCs.

FIGURE 6

qRT-PCR analyses was used to determine whether SFK inhibition effects GDNF-regulated expression of Bcl6b, Erm, and Lhx1. Cultured SSCs were deprived of GDNF for 18 h (O/N-GDNF, yellow bars) followed by 4 h of GDNF replacement (4 h + GDNF, blue bars) with the addition of Me2SO (control), or 100 nm SU6656 (red bars). Relative mRNA expression levels for each gene were normalized to that of ribosomal protein S2 and are presented as -fold change compared with the O/N-GDNF treatment (yellow bars). Data are the mean ± S.E. for three independent experiments. The asterisk denotes significant difference (p ≤ 0.05) between O/N-GDNF and 4 h + GDNF. The number symbol (#) denotes significant difference (p ≤ 0.01) between 4 h + GDNF and 4 h + GDNF + SU6656.