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. 2014 Jun 27;14:59. doi: 10.1186/1472-6750-14-59

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Copyright © 2014 Pereira et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic representation of the diverse AnPGI heterologous expression constructs evaluated in this study: A) constructs used for protein targeting experiments and B) constructs used to analyze the effect of fusion tags on protein expression. p35S, double enhanced 35S promoter from Cauliflower Mosaic Virus 35S gene; tCUP, translation enhancer from the tobacco cryptic upstream promoter; nos, nopaline synthase transcription terminator; Pr1b, tobacco pathogenesis related 1b protein secretory signal peptide; C-myc, detection/purification tag; KDEL, endoplasmic reticulum retrieval tetrapeptide; CTPP, vacuole sorting peptide; RuBisCo T.P., rubisco small subunit transit peptide; HFBI, hydrophobin I; ELP, elastin-like polypeptide. Schematic not drawn to scale.