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. 2014 Jun 28;15:12. doi: 10.1186/1471-2091-15-12

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Copyright © 2014 Dirkx et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sucrose is unable to support the growth of T. vaginalis. TY medium supplemented with maltose (0.5% by weight; TYM), sucrose (0.5% by weight; TYS), or that had no added sugar (TY) was prepared. T. vaginalis was grown for 40 hr in TYM. The cells were collected, washed with TY medium and then used to inoculate this same medium, TYM, or TYS at an initial density of 1 x 10⁵ cells/ml. The cell density was then recorded after 16, 24, 40 and 48 hr of growth. Only TYM medium supported robust growth of T. vaginalis, with a decrease in motile organisms noted by 48 hr in both TY and TYS media. The results shown are the mean ± standard error for four independent determinations.