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Analysis of purified recombinant T. vaginalis β-fructofuranosidase by polyacrylamide gel electrophoresis under denaturing conditions. The T. vaginalis β-fructofuranosidase encoded by TVAG_254130 was expressed in E. coli and purified as described under ‘Methods’. Aliquots corresponding to ~ 25 μg of crude cell extract (Extract) or column flow through (Flow through) were analyzed along with ~ 5 μg of purified β-fructofuranosidase (Purified).
