Table 1.
Glucose/fructose formed (nmol/min/mg) | ||
---|---|---|
|
T. vaginalis |
P. hominis |
(-) Sucrose |
38.5 ± 1.4 |
52.4 ± 4.6 |
(+) Sucrose | 56.0a ± 2.3 | 172a ± 13.8 |
aSignificantly different from value obtained without added sucrose (p < 0.001, Student’s t test).
Extracts were prepared from samples of T. vaginalis and P. hominis grown and collected as described under ‘Methods’. To determine the ability of cell extracts to degrade sucrose, aliquots of cell extract were incubated with (+) or without (-) 250 mM sucrose and the glucose/fructose produced was measured by coupled enzyme assay. Data are expressed per milligram of soluble trichomonad protein. The results shown are the mean ± standard error for five (T. vaginalis) or three (P. hominis) independent experiments.