Table 2.

Kinetic properties of recombinant T. vaginalis β-fructofuranosidase

Substrate	K m (mM)	V max (μmol/min/mg)	Turnover number (s -1 ) †
Sucrose	20.6 ± 2.2	1576 ± 48	1156 ± 36
Raffinose	5.5 ± 0.9	368 ± 16	270 ± 12

^†Based upon a calculated molecular weight of 44 kDa for the recombinant protein.

Purified β-fructofuranosidase (~27 nM, final concentration) was incubated under standard assay conditions with the indicated concentrations of sucrose or raffinose and the initial rate of sugar hydrolysis was measured. Kinetic parameters were determined as described under ‘Methods’. For each substrate, the results shown are the mean ± standard error for three independent experiments.