Figure 1.

Identification of RhoA transgenic founder lines. (a) Schematic of transgenic vector showing inserted HA-tagged human RhoA cDNA and locations of primers used for genotyping by PCR. (b) Protein derived from whole hearts isolated from nontransgenic (NTG) and transgenic (TG) F₁ mice from the 4 founder lines (V3, V12, V13, and V25) was immunoblotted with anti-HA antibody, and bands were quantitated by densitometry. (c) Ventricular protein from 3 nontransgenic and 3 transgenic F₁ mice and 3 nontransgenic and 3 transgenic F₂ mice from the V12 founder line was immunoblotted with anti-HA antibody. A contaminating band at the approximate molecular weight of the HA-RhoA protein band was consistently observed in NTG lanes.