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. 1999 Jul 1;104(1):103–113. doi: 10.1172/JCI3042

Figure 6.

Figure 6

Influence of plasma amino acids on CML formation by myeloperoxidase. The complete myeloperoxidase system (Complete; 30 nM myeloperoxidase, 300 ng/mL glucose oxidase, 100 μg/mL glucose) was incubated for 16 hours at 37°C in buffer A supplemented with 1 mg/ml RNase A, 260 μM L-glutamate, 210 μM L-alanine, 200 μM L-serine, 175 μM glycine, 165 μM L-valine, 100 μM L-proline, and 100 μM L-lysine. The reaction mixture was then subjected to analysis for CML by negative-ion electron capture GC/MS with selected ion monitoring. Sodium azide (Azide; 5 mM) or catalase (Cat; 400 nM) was added when indicated. The bottom 3 bars represent control reactions containing RNase A alone or supplemented with catalase or glucose oxidase. G, glucose; GO, glucose oxidase; MPO, myeloperoxidase.