Skip to main content
. 1999 Jul 1;104(1):67–72. doi: 10.1172/JCI6438

Figure 2.

Figure 2

(a) Western blot analysis of PC1 and PC2 protein expression in human islets cultured for 48 hours in the presence or absence of IL-1β (50 U/mL), IFN-γ (1,000 U/mL), or IL-1β plus IFN-γ. (b) Competitive PCR analysis of PC1 and PC2 mRNA expression in control and cytokine-cultured (IL-1β + IFN-γ, 48 hours) human islets. Lanes 1–4 contain the same amount of cDNA from the same human islet preparation and a decreasing amount of competitor DNA (0.5, 0.125, 0.03, and 0.0075 amol, respectively). Competitor DNA for PC2 was prepared from rat cDNA for PC1 from human cDNA. After amplification, PC1 products were directly run on 2% agarose-ethidium bromide gel, whereas PC2 products were digested with RsaI before separation. Competitor rat PC2 appears as 2 bands (151 and 65 bp) after RsaI restriction, but human PC2 remains intact. The figures are representative of 3 independent experiments.