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. 1999 Aug 1;104(3):281–290. doi: 10.1172/JCI6656

Figure 3.

Figure 3

Absence of anti-Gal–producing cells in GalT+/+→GalT–/– mixed chimeras (19–20 weeks after BMT). (a) ELISPOT detection of anti-Gal–producing (IgM + IgG) cells. Spleen cells (SPL), BMCs (BM), and peritoneal cavity cells (Per C), prepared from normal and conditioned GalT–/– mice, normal GalT+/+ mice, and mixed chimeras 8 days after immunization with rabbit erythrocytes, were used in ELISPOT assay. The frequency of anti-Gal–producing cells was determined as average of red plaque number in triplicate wells of serially diluted cells. The results shown are the average values ± SEM for the individual groups. Number of animals in each group: normal GalT–/– mice, n = 4; normal GalT+/+ mice, n = 4; conditioned GalT–/– mice, n = 5; GalT+/+→GalT–/– mixed chimeras, n = 7 (results are combined for recipients of 20 × 106, 7.5 × 106, and 1 × 106 BMCs). *P < 0.05 compared with normal control GalT–/– mice and similarly conditioned GalT–/– control mice that did not receive BMT. There was no statistically significant difference between mixed chimeras and normal GalT+/+ control mice in any tissue. (b) Serum levels of anti-Gal antibodies after immunization with Gal-bearing xenogeneic cells. Normal and conditioned GalT–/– mice, normal GalT+/+ mice, and mixed chimeras were immunized with rabbit erythrocytes, and serum anti-Gal levels were measured by ELISA assay 8 days after immunization. Average values ± SEM for the individual groups are shown. Number of animals in each group: normal GalT–/– mice, n = 5; conditioned GalT–/– mice, n = 4; mixed chimeras, n = 13 (results are combined for recipients of 20 × 106, 7.5 × 106, and 1 × 106 BMCs); normal GalT+/+ mice, n = 5. *P < 0.05 compared with normal control GalT–/– mice and similarly conditioned GalT–/– control mice that did not receive BMT. There was no statistical difference between mixed chimeras and normal GalT+/+ control mice at any serum dilution in either anti-Gal IgM or IgG levels.