Figure 4.

Absence of anti-Gal–producing cells in mixed chimeras 2 weeks after BMT (ELISPOT detection of anti-Gal IgM/IgG–producing cells). Spleen cells from conditioned GalT^–/– mice receiving 20 × 10⁶ GalT^+/+ BMCs (mixed chimeras), from conditioned GalT^–/– mice receiving 20 × 10⁶ 30 Gy–irradiated GalT^+/+ BMCs (nonchimeras), and from normal GalT^+/+ mice were used in ELISPOT assay. The frequency of anti-Gal–producing cells was determined as the average of red plaque numbers in triplicate wells of 8 × 10⁵ cells. Average values ± SEM for the individual groups are shown. Each point represents an individual mouse. *P < 0.05 compared with conditioned GalT^–/– mice receiving irradiated BMCs.