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PDTC inhibits upregulation of rTLR4 expression in vitro in response to IL-1β or LPS. CMECs and NRVMs were exposed to LPS or cytokines in the presence or absence of PDTC as indicated. This Western blot is representative of 3 independent experiments and illustrates a 96-kDa band consistent with TLR4. CMECs were treated for 36 hours, and NRVMs for 48 hours, with LPS, cytokines, or the NF-κB inhibitor PDTC, as indicated.
