Figure 1.

Construction of hBLTR transgene and screening of hBLTR TG mice. (a) Illustration of the pGL-CD11b-hBLTR construct. Restriction sites used for cloning, and sense (right-facing arrows) and antisense (left-facing arrows) primers used for PCR, are indicated. Nucleotide sequences of the primers corresponding to the hBLTR are underlined. (b) pGL-CD11b-hBLTR and mock (pGL) plasmids were transiently transfected into HEK293 cells, and intracellular calcium was mobilized by addition of LTB₄ (100 nM). Genomic DNA isolated from potential founder mice (c) and F1 generations (d) were analyzed by PCR using primers specific for the CD11b-hBLTR transgene construct. Molecular sizes of expected PCR products are indicated by arrows.