Figure 2.
Overexpression of hBLTR selectively enhanced PMN infiltration in hBLTR TG mice. (a) BLTR-III (sense; right-facing arrow) and BLTR-VII (antisense; left-facing arrow) primers used for amplifying human and mouse BLTR messages are indicated. (b) BLTR (lanes 2 and 4) and GAPDH (lanes 5 and 6) expression in nTG (lanes 2 and 5) and hBLTR TG (lanes 4 and 6) mice was analyzed by RT-PCR. RT-PCR controls (minus RT) are shown in lane 1 (nTG) and lane 3 (TG). The expected molecular size for BLTR at approximately 0.5 kb is indicated by an arrow. The relative intensities of BLTR messages normalized by GAPDH (BLTR/GAPDH) are reported. (c) LTB4 (1 μg), arachidonic acid (10 μg), or PMA (100 ng) was applied topically to the right ears of nTG and TG mice. Left ears received vehicle alone (acetone). Ear skin biopsy samples were taken for MPO analysis. Data were expressed as ratio (TG/nTG) of dermal leukocyte infiltration and (inset) fold increase above vehicle control (*P < 0.01; n = 7 for nTG and n = 10 for TG-hBLTR).